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Plant Biotech J:浙江大学李霞研究组发现GmmiR156b调控大豆理想株型和高产

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摘要 : 近日,国际植物学权威期刊《Plant Biotechnology Journal》在线发表了浙江大学药学院李霞教授团队和与中国科学院遗传与发育生物学研究所童依平研究组、验证手机自动送彩金大学刘宝辉和孔凡江教授研究组合作的一篇研究论文

近日,国际植物学权威期刊《Plant Biotechnology Journal》在线发表了浙江大学药学院李霞教授团队和与中国科学院遗传与发育生物学研究所童依平研究组、验证手机自动送彩金大学刘宝辉和孔凡江教授研究组合作的一篇研究论文,研究论文题为“GENEtic improvement of the shoot architecture and yield in soybean plants via the manipulation of GmmiR156b”。研究揭示了GmmiR156b调控大豆株型的分子机制,为大豆的株型改良和高产提供重要的理论依据。博士生孙政玺为论文第一作者,李霞研究员、童依平研究员、刘宝辉和孔凡江教授为论文通讯作者。

大豆是植物蛋白和植物油最重要的来源之一。随着我国人民生活方式的改变,对大豆的需求量日益增加,我国对国外大豆的依存度也不断提高,致使大豆安全受到严重威胁。因此,培育高产优质大豆迫在眉睫。株型是决定大豆高产的一个最重要性状,其中分枝是大豆株型的主要要素。自1990年代以来,通过优化大豆株型来培育高产大豆一直是育种家及科研工作者的目标,但迄今进展有限,调控大豆分枝和理想株型的主效基因也未见报道。本项研究发现GmmiR156b是调控大豆分枝和理想株型的一个关键基因,过表达GmmiR156b可使大豆具有植株高大、多分枝和高产的特点,是大豆理想株型的一种。田间试验结果显示,过表达GmmiR156b显著增加大豆的分枝数目、主茎节数、主茎的粗度和三出复叶数目,但不影响大豆的株高;过表达GmmiR156b大幅度增加单株荚果的数量、种子变大,单株产量可提高46–63%;此外,通过基因表达模式分析和拟南芥异源互补实验确定了GmmiR156b影响大豆分枝的主效靶基因是GmSPL9d;最后,通过原位杂交以及体内和体外的蛋白质互作实验发现GmSPL9d与调控茎尖和侧生分生组织关键调控因子GmWUS直接互作。该研究首次明确了GmmiR156b是调控大豆理想株型和高产的主效基因,揭示了调控植物分枝形成和发育的miR156b-SPL-WUS的新机制, 也为培育高产大豆提供了具有重要育种价值的基因资源和理论依据。

原文链接:

Genetic improvement of the shoot architecture and yield in soybean plants via the manipulation of GmmiR156b

原文摘要:

The optimization of plant architecture in order to breed high‐yielding soybean cultivars is a goal of researchers. Tall plants bearing many long branches are desired, but only modest success in reaching these goals has been achieved. MicroRNA156 (miR156)‐SQUAMOSA PROMOTER BINDING PROTEIN‐LIKE (SPL) gene modules play pivotal roles in controlling shoot architecture and other traits in crops like rice and wheat. However, the effects of miR156‐SPL modules on soybean architecture and yield, and the molecular mechanisms underlying these effects, remain largely unknown. In this study, we achieved substantial improvements in soybean architecture and yield by overexpressing GmmiR156b. Transgenic plants produced significantly increased numbers of long branches, nodes, and pods, and they exhibited an increased 100‐seed weight, resulting in a 46–63% increase in yield per plant. Intriguingly, GmmiR156boverexpression had no significant impact on plant height in a growth room or under field conditions; however, it increased stem thickness significantly. Our data indicate that GmmiR156b modulates these traits mainly via the direct cleavage of SPL transcripts. Moreover, we found that GmSPL9d is expressed in the shoot apical meristem and axillary meristems of soybean, and that GmSPL9d may regulate axillary bud formation and branching by physically interacting with the homeobox gene WUSCHEL (WUS), a central regulator of axillary meristem formation. Together, our results identifyGmmiR156b as a promising target for the improvement of soybean plant architecture and yields, and they reveal a new and conserved regulatory cascade involving miR156‐SPL‐WUS that will help researchers decipher the genetic basis of plant architecture.

doi:10.1111/pbi.12946

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